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propidium iodide ao pi  (Thermo Fisher)
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Co-administration of gemcitabine and BD B10 induced apoptosis pathway in Panc1 cells. Apoptosis was analysed using multiple methods: <t>(a)</t> <t>AO/PI</t> doubling staining was applied to assess morphological changes in Panc1 cells following each treatment, using fluorescence microscopy, n = 3. Images were captured at 100x magnification (scale bar = 100 µm). (b) The proportion of live (green) and dead (red) cells was quantified using ImageJ and normalised to actual cell counts to analyse cell distribution. (c) Diagram of different apoptotic stages: viable cells, early apoptotic cells, late apoptotic cells, and dead/naked nuclei cells. (d) Annexin V-FITC/PI assay was applied to determine the distribution of different stages of apoptotic cells in Panc1 cells using flow cytometry, n = 4. (e) The percentage of each stage of Panc1 cells was statistically quantified by the iQueForecyt software to analyse cell distribution. Data were analysed using GraphPad Prism, with results expressed as mean ± SEM. Comparisons between treatment groups were evaluated using one-way ANOVA to determine statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001).
Propidium Iodide Ao Pi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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acridine orange propidium iodide ao pi assay  (Thermo Fisher)
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Co-administration of gemcitabine and BD B10 induced apoptosis pathway in Panc1 cells. Apoptosis was analysed using multiple methods: <t>(a)</t> <t>AO/PI</t> doubling staining was applied to assess morphological changes in Panc1 cells following each treatment, using fluorescence microscopy, n = 3. Images were captured at 100x magnification (scale bar = 100 µm). (b) The proportion of live (green) and dead (red) cells was quantified using ImageJ and normalised to actual cell counts to analyse cell distribution. (c) Diagram of different apoptotic stages: viable cells, early apoptotic cells, late apoptotic cells, and dead/naked nuclei cells. (d) Annexin V-FITC/PI assay was applied to determine the distribution of different stages of apoptotic cells in Panc1 cells using flow cytometry, n = 4. (e) The percentage of each stage of Panc1 cells was statistically quantified by the iQueForecyt software to analyse cell distribution. Data were analysed using GraphPad Prism, with results expressed as mean ± SEM. Comparisons between treatment groups were evaluated using one-way ANOVA to determine statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001).
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Co-administration of gemcitabine and BD B10 induced apoptosis pathway in Panc1 cells. Apoptosis was analysed using multiple methods: <t>(a)</t> <t>AO/PI</t> doubling staining was applied to assess morphological changes in Panc1 cells following each treatment, using fluorescence microscopy, n = 3. Images were captured at 100x magnification (scale bar = 100 µm). (b) The proportion of live (green) and dead (red) cells was quantified using ImageJ and normalised to actual cell counts to analyse cell distribution. (c) Diagram of different apoptotic stages: viable cells, early apoptotic cells, late apoptotic cells, and dead/naked nuclei cells. (d) Annexin V-FITC/PI assay was applied to determine the distribution of different stages of apoptotic cells in Panc1 cells using flow cytometry, n = 4. (e) The percentage of each stage of Panc1 cells was statistically quantified by the iQueForecyt software to analyse cell distribution. Data were analysed using GraphPad Prism, with results expressed as mean ± SEM. Comparisons between treatment groups were evaluated using one-way ANOVA to determine statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001).
Acridine Orange Propidium Iodide Ao Pi Viability Dye, supplied by Logos Biosystems Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dye propidium iodide pi  (Keygen Biotech)
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Co-administration of gemcitabine and BD B10 induced apoptosis pathway in Panc1 cells. Apoptosis was analysed using multiple methods: <t>(a)</t> <t>AO/PI</t> doubling staining was applied to assess morphological changes in Panc1 cells following each treatment, using fluorescence microscopy, n = 3. Images were captured at 100x magnification (scale bar = 100 µm). (b) The proportion of live (green) and dead (red) cells was quantified using ImageJ and normalised to actual cell counts to analyse cell distribution. (c) Diagram of different apoptotic stages: viable cells, early apoptotic cells, late apoptotic cells, and dead/naked nuclei cells. (d) Annexin V-FITC/PI assay was applied to determine the distribution of different stages of apoptotic cells in Panc1 cells using flow cytometry, n = 4. (e) The percentage of each stage of Panc1 cells was statistically quantified by the iQueForecyt software to analyse cell distribution. Data were analysed using GraphPad Prism, with results expressed as mean ± SEM. Comparisons between treatment groups were evaluated using one-way ANOVA to determine statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001).
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dna binding dye propidium iodide pi  (Keygen Biotech)
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Co-administration of gemcitabine and BD B10 induced apoptosis pathway in Panc1 cells. Apoptosis was analysed using multiple methods: <t>(a)</t> <t>AO/PI</t> doubling staining was applied to assess morphological changes in Panc1 cells following each treatment, using fluorescence microscopy, n = 3. Images were captured at 100x magnification (scale bar = 100 µm). (b) The proportion of live (green) and dead (red) cells was quantified using ImageJ and normalised to actual cell counts to analyse cell distribution. (c) Diagram of different apoptotic stages: viable cells, early apoptotic cells, late apoptotic cells, and dead/naked nuclei cells. (d) Annexin V-FITC/PI assay was applied to determine the distribution of different stages of apoptotic cells in Panc1 cells using flow cytometry, n = 4. (e) The percentage of each stage of Panc1 cells was statistically quantified by the iQueForecyt software to analyse cell distribution. Data were analysed using GraphPad Prism, with results expressed as mean ± SEM. Comparisons between treatment groups were evaluated using one-way ANOVA to determine statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001).
Dna Binding Dye Propidium Iodide Pi, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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propidium iodide pi dna dyes  (Thermo Fisher)
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Co-administration of gemcitabine and BD B10 induced apoptosis pathway in Panc1 cells. Apoptosis was analysed using multiple methods: <t>(a)</t> <t>AO/PI</t> doubling staining was applied to assess morphological changes in Panc1 cells following each treatment, using fluorescence microscopy, n = 3. Images were captured at 100x magnification (scale bar = 100 µm). (b) The proportion of live (green) and dead (red) cells was quantified using ImageJ and normalised to actual cell counts to analyse cell distribution. (c) Diagram of different apoptotic stages: viable cells, early apoptotic cells, late apoptotic cells, and dead/naked nuclei cells. (d) Annexin V-FITC/PI assay was applied to determine the distribution of different stages of apoptotic cells in Panc1 cells using flow cytometry, n = 4. (e) The percentage of each stage of Panc1 cells was statistically quantified by the iQueForecyt software to analyse cell distribution. Data were analysed using GraphPad Prism, with results expressed as mean ± SEM. Comparisons between treatment groups were evaluated using one-way ANOVA to determine statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001).
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Co-administration of gemcitabine and BD B10 induced apoptosis pathway in Panc1 cells. Apoptosis was analysed using multiple methods: (a) AO/PI doubling staining was applied to assess morphological changes in Panc1 cells following each treatment, using fluorescence microscopy, n = 3. Images were captured at 100x magnification (scale bar = 100 µm). (b) The proportion of live (green) and dead (red) cells was quantified using ImageJ and normalised to actual cell counts to analyse cell distribution. (c) Diagram of different apoptotic stages: viable cells, early apoptotic cells, late apoptotic cells, and dead/naked nuclei cells. (d) Annexin V-FITC/PI assay was applied to determine the distribution of different stages of apoptotic cells in Panc1 cells using flow cytometry, n = 4. (e) The percentage of each stage of Panc1 cells was statistically quantified by the iQueForecyt software to analyse cell distribution. Data were analysed using GraphPad Prism, with results expressed as mean ± SEM. Comparisons between treatment groups were evaluated using one-way ANOVA to determine statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001).

Journal: Frontiers in Pharmacology

Article Title: Enhancement of gemcitabine toxicity and specificity through PI3K/Akt/Nrf2 pathway inhibition in pancreatic cancer

doi: 10.3389/fphar.2026.1724989

Figure Lengend Snippet: Co-administration of gemcitabine and BD B10 induced apoptosis pathway in Panc1 cells. Apoptosis was analysed using multiple methods: (a) AO/PI doubling staining was applied to assess morphological changes in Panc1 cells following each treatment, using fluorescence microscopy, n = 3. Images were captured at 100x magnification (scale bar = 100 µm). (b) The proportion of live (green) and dead (red) cells was quantified using ImageJ and normalised to actual cell counts to analyse cell distribution. (c) Diagram of different apoptotic stages: viable cells, early apoptotic cells, late apoptotic cells, and dead/naked nuclei cells. (d) Annexin V-FITC/PI assay was applied to determine the distribution of different stages of apoptotic cells in Panc1 cells using flow cytometry, n = 4. (e) The percentage of each stage of Panc1 cells was statistically quantified by the iQueForecyt software to analyse cell distribution. Data were analysed using GraphPad Prism, with results expressed as mean ± SEM. Comparisons between treatment groups were evaluated using one-way ANOVA to determine statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001).

Article Snippet: Subsequently, the cells were stained with a mixture of Acridine orange and propidium iodide (AO/PI) at 10 μg/mL (AO: 300910250, Thermo Fisher Scientific; PI: P4864, Sigma) in the dark for 5 min at 37 °C.

Techniques: Staining, Fluorescence, Microscopy, Flow Cytometry, Software